Effective Cleaning Of Pipettes

December 05, 2012

 Chemical, water or other analysis requiring exact measurement and dispensing of liquid reagents relies on the use of pipettes. Some pipettes are disposable, however, reusable glass pipettes are preferred because they are typically of higher quality and are calibrated more exactly. The main disadvantage of using glass pipettes is the difficulty of cleaning them between users.

Some pipette cleaners are simple plastic jugs that fill and siphon water several times. Cleaning is accomplished by means of soaking and flushing. After this process, pipettes are typical placed in an oven for drying. This process can take hours and requires handling of wet pipettes. As a result, the tips are easily broken, rendering the pipettes useless. Another cleaning method is to use a laboratory glassware washer. Miele lab glassware washers equipped with pipette injector baskets and specified cleaning agents are ideally suited for the task and provide repeatable and analytically clean results.

When cleaning pipettes, Miele injection baskets provide remarkably clean results for hard-to-reach interiors. Each pipette is placed tip down in a protective plastic holder, where water and detergent are injected providing thorough cleaning. A heated DI rinse follows to ensure complete removal of trace residues. If HEPA-filtered drying is used, forced hot air will circulate through the pipettes, providing complete drying.

Sources: Laboratory Network.com
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Postdoctoral Fellowship in Microbiology and Molecular Biology

December 02, 2012

A postdoctoral position is open for a project aiming to analyze bacterial and fungal scalp microbiota in healthy subjects and in patients with skin diseases, such as dandruff and seborrheic dermatitis.

The ideal candidate will have experience in Molecular Biology methods, including PCR, real time-PCR, cloning and DNA sequencing; as well as Bioinformatics. Written and spoken fluency in English is essential. 

The selected candidate will conduct experiments, analyze and discuss results with international collaborators; and is also expected to provide support to students, and to assist in general lab activities.  

The project will be developed at Universidade Federal do ABC (UFABC), in Santo Andre, Sao Paulo, Brazil, under the supervision of Dr. Luciana Campos Paulino.

The position is initially offered for 12 months, with the possibility to be extended for another 12 months. Monthly salary: R$ 6,200.00.

The opening is available for Brazilian and foreign candidates (holding the required visa for research activities in Brazil). 

Applicants should send a letter of interest; curriculum vitae; and two letters of recommendation to luciana.paulino@ufabc.edu.br. Deadline for submissions is December, 14th 2012.
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Miami 2013 Winter Symposium: The Molecular Basis of Metabolism and Nutrition

October 13, 2012

This meeting seeks to integrate existing knowledge on molecular mechanisms of metabolism and metabolic disease with emerging knowledge on the influence of hormones, nutrients and microbial flora that influence systemic metabolism. Transient nutritional exposures during critical developmental periods can induce permanent alterations in epigenetic regulation that program metabolic and other biological networks, with lifelong consequences. There is also growing molecular understanding of the role of processes, such as ER stress response, autophagy, mitochondrial dysfunction, inflammation and hormone signaling. The interaction of nutrition, epigenetics and human health is a rapidly evolving area of research with many fundamental outstanding questions and opportunities to translate basic science discoveries to clinical and public health applications.

The 2013 Miami Winter Symposium will bring together leaders the field to discuss advances in our molecular understanding of obesity, calorie restriction, diabetes, aging and the microbiome as they relate to human nutrition.
  • Organizers

    • University Biochemistry & Molecular Biology Foundation Inc.
    • University of Miami
    • International Union of Biochemistry and Molecular Biology
    • Scripps Florida
    • Nature Biotechnology
    • Nature Cell Biology
    • Nature Medicine
  • Date

    February 10-13, 2013
  • Venue

    JW Marriott Marquis Miami, Miami, FL, USA

    For more information and to register, visit:
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Genomics Research Europe

August 23, 2012

Advances in qPCR, which will showcase new developments in qPCR technology and its wide range of applications. Sessions will focus on improvements to qPCR design, the acquisition of accurate data, and efficient data analysis. There will also be presentations demonstrating the role of qPCR in molecular diagnostics and the detection of tumour cells.

RNAi & miRNA track will detail the cutting edge research taking place in the two fields with a particular emphasis on the complex roles microRNA play in cancer and their regulation. The latest developments in both therapeutics and delivery of RNAi based medicines will also be discussed to help map out the commercial trends occurring in this field.

The other tracks include Epigenetics and Agrigenomics. Registered delegates will have access to all four meetings ensuring a very cost-effective trip. For more information on each track visit our website.

To view the full speaker line-up and to view a full programme, visit our website by clicking on the link below.

Contact Kirit Shan
Tel: +44 (0) 1787 315127
E-mail: k.shah@selectbiosciences.com

04 Sep 2012 to 05 Sep 2012

for more info click here

Event Location:
Sheraton Frankfurt Airport Hotel, Hugo-Eckener-Ring 15 Rhein, Main Airport, 60549, Frankfurt, Germany

Sources: http://www.labroots.com/app/events/view/5734
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DNA Removal From Bioprocess Purification Processes

February 20, 2012

Many new biological drug products produced using recombinant DNA technology, such as monoclonal antibodies, are produced in cell culture. The host cell produces the therapeutic proteins that are often secreted outside the cell into the surrounding culture medium. Purification of the therapeutic proteins requires separation of the host cell mass from the extracellularly secreted proteins followed by an extensive series of downstream purification steps. Production of these therapeutic proteins is regulated by the Food and Drug Administration (FDA) Center for Biologics Evaluation and Research (CBER). CBER provides guidelines for biologically produced drugs including suggested limitations on final product impurities. Because therapeutic proteins such as monoclonal antibodies are produced in cell culture, impurities can result from the host cells, or cell substrates. Examples of these impurities are host cell protein and host cell DNA. In, Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use, February 28, 1997, CBER states that "It is suggested that, wherever possible, the final product contain no more than 100 pg cellular DNA per dose."
In any purification process it is desirable to remove impurities as early in the process as possible. This 3M Purification Application Brief addresses removal of DNA using cellulosic depth filters. Depth filtration is often employed for the first purification step, separation of cell mass from therapeutic proteins.

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